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KMID : 0386319980310020095
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Korean Leprosy Bulletin
1998 Volume.31 No. 2 p.95 ~ p.103
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Molecular Biological Studies for Detection of Mycobacterium leprae in Long-term Smear Negative Leprosy Patients
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Shin Soon-Shick
Kim Jong-Bong
Kim Sung-Hwa
Chae Gue-Tae
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Abstract
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Our research was carried out to confirm that the PGL1-ELISA test and
DNA PCR can be useful methods for early detection of M. leprae bacilli in smear
negative leprosy patients. M. leprae bacilli were screened by PCR from skin biopsy
spectrums in patients with PGL1-IgM antibody positive cases(MB : 28, PB
: 3) and antibody negative cases(MB : 11, PB : 8). M. leprae bacilli were confirmed by
PCR in 7(18%) of the MB patients and none of the PB patients.
There was a significant relationship between the PGL1 antibody and
PCR; MB patients who were PCR+ had a mean antibody titer of 0.699±0.202 O. D.
compared to MB patients who were PCR-(mean 0.475±0.421 O. D.) and PCR-PB
patients (mean 0.118±0.133 O. D.).
There results suggest that the PGL1-ELISA test and PCR can be useful
tools for the screening of M. leprae bacilli and, possibly relapse in leprosy.
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